Cloned (Comment) | Organism |
---|---|
BcOS4 gene, DNA and amino acid sequence determination and analysis | Botrytis cinerea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction and complementation of the BcOS4 deletion mutant. Comparison of mycelial growth rate of the wild-type strain 38B1, the BcOS4 deletion mutant DELTABcOs4-E8, and the complemented strain DELTABcOs4-E8-C23 on potato dextrose agar and minimal medium, overview The BcOS4 deletion mutant is significantly impaired in vegetative growth and conidial formation. All the defects are restored by genetic complementation of the BcOS4 deletion mutant with the wild-type BcOS4 gene. Phenotype of the BcOS4 deletion mutant, overview. The mutant strain exhibits increased sensitivity to the dicarboximide fungicide iprodione and the phenylpyrrole fungicide fludioxonil | Botrytis cinerea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Botrytis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + BcSak1 | Botrytis cinerea | BcSak1 is a putative downstream component of BcOs4 | ADP + phosphorylated BcSak1 | - |
? | |
ATP + BcSak1 | Botrytis cinerea 38B1 | BcSak1 is a putative downstream component of BcOs4 | ADP + phosphorylated BcSak1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botrytis cinerea | I7FWY1 | i.e. Botryotinia fuckeliana, strain 38B1 is isolated from a grape in California, USA, and deposited to the China Microbiological Culture Collection Center, accession number CGMCC no. 4006 | - |
Botrytis cinerea 38B1 | I7FWY1 | i.e. Botryotinia fuckeliana, strain 38B1 is isolated from a grape in California, USA, and deposited to the China Microbiological Culture Collection Center, accession number CGMCC no. 4006 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypha | - |
Botrytis cinerea | - |
mycelium | - |
Botrytis cinerea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + BcSak1 | - |
Botrytis cinerea | ADP + phosphorylated BcSak1 | - |
? | |
ATP + BcSak1 | BcSak1 is a putative downstream component of BcOs4 | Botrytis cinerea | ADP + phosphorylated BcSak1 | - |
? | |
ATP + BcSak1 | - |
Botrytis cinerea 38B1 | ADP + phosphorylated BcSak1 | - |
? | |
ATP + BcSak1 | BcSak1 is a putative downstream component of BcOs4 | Botrytis cinerea 38B1 | ADP + phosphorylated BcSak1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BcOs4 | - |
Botrytis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Botrytis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Botrytis cinerea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Botrytis cinerea |
General Information | Comment | Organism |
---|---|---|
evolution | mitogen-activated protein kinase kinase kinase gene BcOS4 in Botrytis cinerea is homologous to Saccharomyces cerevisiae SSK2/SSK22 | Botrytis cinerea |
malfunction | the BcOS4 deletion mutant is significantly impaired in vegetative growth and conidial formation. The mutant exhibits increased sensitivity to the osmotic, oxidative stresses and to the fungicides iprodione and fludioxonil. BcSak1, a putative downstream component of BcOs4, is not phosphorylated in the mutant. The BcOS4 mutant is unable to infect leaves of rapeseed and cucumber, and grape fruits, although it can cause disease on apple fruits. The mutant strain exhibits increased sensitivity to the dicarboximide fungicide iprodione and the phenylpyrrole fungicide fludioxonil. All the defects are restored by genetic complementation of the BcOS4 deletion mutant with the wild-type BcOS4 gene. Phenotype of the BcOS4 deletion mutant, overview | Botrytis cinerea |
physiological function | mitogen-activated protein kinase kinase kinase BcOs4 is required for vegetative differentiation and pathogenicity in Botrytis cinerea. BcOS4 is involved in vegetative differentiation, virulence, adaption to hyperosmotic and oxidative stresses, and to fungicides | Botrytis cinerea |